Journal: Heliyon
Article Title: circSnd1 promotes atherosclerosis progression through the miR-485-3p/Olr1 signaling pathway
doi: 10.1016/j.heliyon.2023.e17366
Figure Lengend Snippet: circSnd1 targets miR-485-3p to regulate inflammation and lipid transport. A, The expression level of circSnd1 after manipulating the expression of circSnd1 and/or miR-485-3p in ox-LDL-induced macrophages using siRNA, miRNA mimic or inhibitor. B, The expression level of miR-485-3p after the alteration of circSnd1 and/or miR-485-3p in ox-LDL-induced macrophages. C-D, The binding sites of circSnd1 by miR-485-3p and its validation by the luciferase reporter experiment. E, Inflammatory factors were detected by ELISA. F, The protein expression of lipid composition and cholesterol transport indicators (Abca1, Abcg1, Ldlr, Hdlbp, Lp-pla2, and Srebp-1c) and Olr1. *, Compared with normal, # compared with model, & compared with si-circRNA. *, #, &, p < 0.05; **, ##, &&, p < 0.01.
Article Snippet: Primary antibody dilutions (Olr1 (1:1000, DF6522, Affinity), Hdlbp (1:1000, ab133594, Abcam), Ldlr (1:100), Srebp-1c (1:100), Lp-pla2 (1:100), Abcg1 (1:100), Abca1 (1:100), and Gapdh (1:500)) were dropped onto the glass slides and incubated overnight.
Techniques: Expressing, Binding Assay, Luciferase, Enzyme-linked Immunosorbent Assay